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verikine-hs human ifn beta elisa kit  (PBL Assay)


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    PBL Assay verikine-hs human ifn beta elisa kit
    Verikine Hs Human Ifn Beta Elisa Kit, supplied by PBL Assay, used in various techniques. Bioz Stars score: 94/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ifn+beta/VeriKine-HS+Human+IFN+Beta+ELISA+Kit/custom%4041415-1%4010%2E3390%2Fv18080828
    Average 94 stars, based on 142 article reviews
    verikine-hs human ifn beta elisa kit - by Bioz Stars, 2026-09
    94/100 stars

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    FLAG-tag:

    Article Title: Chloroquine enhances MAVS-mediated cytokine induction and alters mitochondrial morphology.
    Article Snippet: .. The antibodies detecting Myc-tag (#2276), MAVS (#3993), pSTAT1 (#9167), STAT1 (#9175), and V5-tag (#13202) were from Cell Signaling Technology; antibody detecting FLAG-tag (F1804) was from Sigma-Aldrich; antibody for IFN-Beta (31410-1) was from PBL Assay Science; antibody for actin (GTX629630) was from GeneTex, and then incubated with corresponding secondary antibodies. .. Signals were detected by Immobilon Western Chemiluminescence HRP Substrate (WBKLS0500, Millipore) using UVP (ChemiDoc-It Imaging system, Analytik) with VisionWorks LS v.8.20 software.

    Article Title: Chloroquine Enhances Mitochondrial Antiviral Signaling-Mediated Cytokine Induction and Alters Mitochondrial Morphology
    Article Snippet: .. The antibodies detecting Myc-tag (#2276), MAVS (#3993), pSTAT1 (#9167), STAT1 (#9175), and V5-tag (#13202) were from Cell Signaling Technology; antibody detecting FLAG-tag (F1804) was from Sigma-Aldrich; antibody for IFN-beta (31410-1) was from PBL Assay Science; antibody for actin (GTX629630) was from GeneTex and then incubated with corresponding secondary antibodies. .. Signals were detected by Immobilon Western Chemiluminescence HRP Substrate (WBKLS0500, Millipore) using UVP (ChemiDoc-It Imaging System, Analytik) with VisionWorks LS v.8.20 software.

    Incubation:

    Article Title: Chloroquine enhances MAVS-mediated cytokine induction and alters mitochondrial morphology.
    Article Snippet: .. The antibodies detecting Myc-tag (#2276), MAVS (#3993), pSTAT1 (#9167), STAT1 (#9175), and V5-tag (#13202) were from Cell Signaling Technology; antibody detecting FLAG-tag (F1804) was from Sigma-Aldrich; antibody for IFN-Beta (31410-1) was from PBL Assay Science; antibody for actin (GTX629630) was from GeneTex, and then incubated with corresponding secondary antibodies. .. Signals were detected by Immobilon Western Chemiluminescence HRP Substrate (WBKLS0500, Millipore) using UVP (ChemiDoc-It Imaging system, Analytik) with VisionWorks LS v.8.20 software.

    Article Title: Chloroquine Enhances Mitochondrial Antiviral Signaling-Mediated Cytokine Induction and Alters Mitochondrial Morphology
    Article Snippet: .. The antibodies detecting Myc-tag (#2276), MAVS (#3993), pSTAT1 (#9167), STAT1 (#9175), and V5-tag (#13202) were from Cell Signaling Technology; antibody detecting FLAG-tag (F1804) was from Sigma-Aldrich; antibody for IFN-beta (31410-1) was from PBL Assay Science; antibody for actin (GTX629630) was from GeneTex and then incubated with corresponding secondary antibodies. .. Signals were detected by Immobilon Western Chemiluminescence HRP Substrate (WBKLS0500, Millipore) using UVP (ChemiDoc-It Imaging System, Analytik) with VisionWorks LS v.8.20 software.

    Recombinant:

    Article Title: Epstein-Barr virus tegument protein BGLF2 in exosomes released from virus-producer cells assists de novo infection
    Article Snippet: Bortezomib (Cat# A10160; AdooQ BioScience, Irvine, CA, USA) was used at a concentration of 100 nM. .. Recombinant human type I IFN alpha (Cat# 11200-1) and IFN beta (Cat# 11415-1) were purchased from PBL Assay Science (Piscataway, NJ, USA). .. For inhibition of exosome secretion, cells were treated with 10 μM GW4869 (Cat# 13127; Cayman Chemical, Ann Arbor, MI, USA).

    Article Title: Innate Immune Response to Influenza Virus at Single-Cell Resolution in Human Epithelial Cells Revealed Paracrine Induction of Interferon Lambda 1
    Article Snippet: .. 297 298 Cytokine stimulation experiments 299 A549 cells were stimulated with recombinant TNF alpha (Gibco), IFN beta (PBL Assay 300 Science), IFN lambda-1 (PBL Assay Science), or different combinations of these 301 on A ugust 2, 2019 by guest http://jvi.asm .org/ D ow nloaded from 15 cytokines for 8 h at 100 U/ml, in EMEM media supplemented with 5% NaHCO3, 302 penicillin/streptomycin (Gibco), L-glutamine (Gibco) and 1% FBS. ..

    Article Title: Dendritic cells Trigger IFN- γ secretion by NK cells independent of IL-12 and IL-18
    Article Snippet: The culture medium used throughout was Iscove’s modified Dulbecco’s medium (IMDM: Gibco BRL). .. Recombinant mouse IL-2 (PeproTech), IFN beta and anti-mouse IFN-β were purchased (pbl Assay Science), and the mouse p40 homodimer was purchased from R&D Systems. .. The following antibodies were purchased from Biolegend: NK1.1-biotin (PK136), NKp46-biotin (29A1.4), CD3-biotin (17A2), CD19-biotin (6D5), MHC-II PerCP/Cy5.5 (AF6-120.1 & M5/114), CD11c BV785 (N418), CD3 FITC, CD19 FITC, Ter119 FITC, NK1.1 647 (PK136), -NKp46 PE/Dazzle (29A1.4), DAPI and -CD16/32 (Ultra-LEAF Purified clone 93).

    Article Title: Epstein–Barr virus tegument protein BGLF2 in exosomes released from virus-producing cells facilitates de novo infection
    Article Snippet: Bortezomib (Cat# A10160; AdooQ BioScience, Irvine, CA, USA) was used at a concentration of 100 nM. .. Recombinant human type I IFN alpha (Cat# 11200-1) and IFN beta (Cat# 11415-1) were purchased from PBL Assay Science (Piscataway, NJ, USA). .. For inhibition of exosome secretion, cells were treated with 10 μM GW4869 (Cat# 13127; Cayman Chemical, Ann Arbor, MI, USA).

    Article Title: Innate Immune Response to Influenza Virus at Single-Cell Resolution in Human Epithelial Cells Revealed Paracrine Induction of Interferon Lambda 1
    Article Snippet: .. A549 cells were stimulated with recombinant TNF-α (Gibco), IFN beta (PBL Assay Science), IFNL1 (PBL Assay Science), or different combinations of these cytokines for 8 h at 100 U/ml in EMEM supplemented with 5% NaHCO 3 , penicillin-streptomycin (Gibco), l -glutamine (Gibco), and 1% FBS. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Maternal Influenza A Virus Infection Induces Antiviral and Immune Dysregulation in the Placenta and Fetus Without Vertical Transmission
    Article Snippet: Tissue lysis and homogenization were performed using an InvitrogenTM ProcartaPlexTM Cell Lysis Buffer (#EPX-99999-000, Thermo Fisher Scientific) with the addition of a protease inhibitor cocktail (Fisher Scientific, #PI78430) using the Precellys Evolution system, as previously described. .. Type I Interferons [IFN; IFN-alpha (IFNα) and IFN-beta (IFNβ)] were quantified using the Cynomolgus IFN-beta and Cynomolgus/Rhesus IFN-alpha ELISA kits (#46415-1 and #46100-1, respectively; PBL Biosciences, Menlo Park, CA, USA). ..



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    Image Search Results


    Type I and II IFNs differ from type III IFN in their regulation of TGF-β expression and the ERK–JNK pathway in renal fibroblasts during kidney fibrosis. (A–C) Primary renal fibroblasts from WT mice were treated for 24 h with 100 ng/ml of various IFNs subtypes (IFN-α, IFN-β, IFN-γ, or IFN-λ2) or PBS. (A and B) Isg15 , Mx1 , Ifit1 , and Tgf-β mRNA levels were assessed by RT-qPCR ( n = 6), and (C) TGF-β protein in the culture supernatants was quantified by ELISA ( n = 4). (D–F) Primary skin fibroblasts from WT mice were treated for 24 h with 100 ng/ml of various IFNs subtypes (IFN-α, IFN-β, IFN-γ, or IFN-λ2) or PBS. (D and E) Isg15 , Mx1 , Ifit1 , and Tgf-β mRNA levels were detected by RT-qPCR ( n = 6), and (F) TGF-β protein in the culture supernatants was measured by ELISA ( n = 4). (G–J) WT, Ifnar –/– , and Ifngr1 −/− mice were subjected to sham or UUO surgery, and kidneys were collected on day 7. n = 6 per group. (G) Representative images and quantitative analysis of fibrotic areas with Masson’s trichrome and PSR staining (scale bars = 50 μm). (H) RT-qPCR analysis of Acta2 , fibronectin, and vimentin mRNA levels in kidneys. TGF-β mRNA and protein levels in kidneys were measured by RT-qPCR (I) and western blot (J). (K and L) Primary kidney fibroblasts were treated with 100 ng/ml IFN-α (K) or IFN-β (L) for the indicated times. (K and L) Western blot analysis of phosphorylated and total ERK and JNK protein levels. Data in A–I are pooled from two independent experiments. Data in J–L are representative of three independent experiments. Data are presented as mean ± SEM. *P < 0.05, ****P < 0.0001, by two-way ANOVA with Tukey’s multiple-comparison test (A–I). ns, no significant difference. Source data are available for this figure: .

    Journal: The Journal of Experimental Medicine

    Article Title: Interferon-λ drives renal fibrosis by coordinating epithelial–fibroblast crosstalk

    doi: 10.1084/jem.20251858

    Figure Lengend Snippet: Type I and II IFNs differ from type III IFN in their regulation of TGF-β expression and the ERK–JNK pathway in renal fibroblasts during kidney fibrosis. (A–C) Primary renal fibroblasts from WT mice were treated for 24 h with 100 ng/ml of various IFNs subtypes (IFN-α, IFN-β, IFN-γ, or IFN-λ2) or PBS. (A and B) Isg15 , Mx1 , Ifit1 , and Tgf-β mRNA levels were assessed by RT-qPCR ( n = 6), and (C) TGF-β protein in the culture supernatants was quantified by ELISA ( n = 4). (D–F) Primary skin fibroblasts from WT mice were treated for 24 h with 100 ng/ml of various IFNs subtypes (IFN-α, IFN-β, IFN-γ, or IFN-λ2) or PBS. (D and E) Isg15 , Mx1 , Ifit1 , and Tgf-β mRNA levels were detected by RT-qPCR ( n = 6), and (F) TGF-β protein in the culture supernatants was measured by ELISA ( n = 4). (G–J) WT, Ifnar –/– , and Ifngr1 −/− mice were subjected to sham or UUO surgery, and kidneys were collected on day 7. n = 6 per group. (G) Representative images and quantitative analysis of fibrotic areas with Masson’s trichrome and PSR staining (scale bars = 50 μm). (H) RT-qPCR analysis of Acta2 , fibronectin, and vimentin mRNA levels in kidneys. TGF-β mRNA and protein levels in kidneys were measured by RT-qPCR (I) and western blot (J). (K and L) Primary kidney fibroblasts were treated with 100 ng/ml IFN-α (K) or IFN-β (L) for the indicated times. (K and L) Western blot analysis of phosphorylated and total ERK and JNK protein levels. Data in A–I are pooled from two independent experiments. Data in J–L are representative of three independent experiments. Data are presented as mean ± SEM. *P < 0.05, ****P < 0.0001, by two-way ANOVA with Tukey’s multiple-comparison test (A–I). ns, no significant difference. Source data are available for this figure: .

    Article Snippet: Primary renal fibroblasts isolated from WT and Ifnlr1 −/− mice were stimulated with or without 100 ng/ml IFN-λ2 (250-33; PeproTech) for 1 h or 24 h. In separate experiments, primary renal and skin fibroblasts from WT mice were treated with 100 ng/ml of IFN-λ2 (250-33; PeproTech), IFN-α (CK83; Novoprotein), IFN-β (HY- P73130 ; MedChemExpress), or IFN-γ (315-05; PeproTech) for different times.

    Techniques: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Staining, Western Blot, Comparison